| Solution Information | help | |
| Enzyme: | Methyl-CpG-binding domain protein 2 | |
| inhibitor: | BDBM89192 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose responses curves for compounds identified as active in a previous set of experiments entitled, "TRFRET-based biochemical primary high throughput screening assay to identify inhibitors of 5-meCpG-binding domain protein 2 (MBD2)-DBD binding to methylated oligonucleotide" (PubChem AID 686964) and "TRFRET-based biochemical high throughput confirmation assay to identify inhibitors of 5-meCpG-binding domain protein 2 (MBD2)-DBD binding to methylated oligonucleotide" (PubChem AID 720531), and were inactive in a set of experiments entitled, "Counterscreen for inhibitors of 5-meCpG-binding domain protein 2 (MBD2): TRFRET-based biochemical high throughput screening assay to identify inhibitors of binding of ubiquitin-like with PHD and ring finger domains 1 (UHRF1) to methylated oligonucleotide" (PubChem AID 720530). This biochemical TR-FRET-based assay employs MBD2-DBD and FAM-labeled 14bp hairpin oligonucleotide containing a symm | |
| If you find an error in this entry please send us an E-mail | ||